Figure 3.Psp68 promoter-GUS analysis in response to abiotic stress. (A) GUS activity in agroinfiltrated leaves. GUS was detected in X-Gluc solution followed by stress treatment. (B) Effect of abiotic stress on the transient expression of Psp68 promoter in agroinfiltrated tobacco leaves. Two days after infiltration with A. tumefaciens (OD _0.7) containing either Psp68 promoter::GUS or CaMV35S::GUS constructs, tobacco leaves were sprayed with 200 mM NaCl, 20% PEG. For cold treatment infiltrated leaves were kept on 4 °C. After treatment, all the samples were used for quantification assay. Infiltrated leaves of WT without treatment (water) used as negative control. Data represent the mean and SD of 4 independent experiments