Figure 5. Analysis of global genome methylation in the F1 progeny of wild type (15D8) and mutant (dcl2 and dcl3) plants exposed to heat and control plants. Y-axis shows units of radioactive incorporation (dpm/µg). Higher incorporation indicates a lower degree of methylation. “C₁” – the progeny of plants grown at normal conditions in F0. “S₁” – the progeny of plants exposed to heat in F0. “+” and “-” indicate exposure to stress or growth in uninduced conditions, respectively. Error bars show standard deviation calculated from 2 technical repeats. Asterisks (*, **) indicate a significant difference between control (-) and stress (+) with the same parental treatment, between different treatments (C₁ vs S₁) or between different mutants, as calculated using a t test (P ≤ 0.05 or P ≤ 0.01, for 1 or 2 asterisks, respectively).