Figure 3. Soybean rust protein PHEP 369 in soybean variety W82 and isoline Rpp1 challenged with rust and BPMV. Rpp1 plants were preinfected with BPMV 1037 (control) or with the denoted BPMV silencing constructs. PHEP 369 was detected with anti-PHEP 369 antibody. One hundred times less total protein from the W82+rust sample was evaluated. Data are from rust challenge 5. Five independent replicates with silencing constructs are shown.