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. 2014 Jan 21;9:e27540. doi: 10.4161/psb.27540

graphic file with name psb-9-e27540-g2.jpg

Figure 2. SERF1 influences the transcriptional response of genes relevant for ionic and osmotic homeostasis in leaves. Expression levels of genes encoding (A) ion channels, (B) transporters, (C) aquaporins and (D) ATPases were measured in leaves of wild-type and serf1 plants subjected to salt stress for 30 min or 3 h (100 mM NaCl). ACTIN (Os03g50885) was used as reference gene. Data represent means ± SE from 3 biological replicates. An asterisk (*) indicates a significant difference between treated and control plants (P ≤ 0.05; the Student t test). In the heat map the gene expression under H2O2 (5 mM), ABA (5 µM) or mannitol (100 mM) treatment is shown in log2FC relative to the control situation with n = 3. The potassium channels SKOR* and SKOR** are encoded by Os04g36740 and Os06g14030, respectively. Os06g46670, Os07g01310, Os09g26160 and Os09g31160 encode GLRs. CaCA* and CaCA** are encoded by Os11g01580 and Os12g42910, respectively. Os07g26640 encodes an aquaporin. FC, fold change; WT, wild type; GLR, glutamate-like receptor; CaCA, calcium cation antiporter.