Fig. 1.

HCMV-induced apoptosis of hNPCs under physiological oxygen tensions. (a) Flow cytometry analyses. (Top) Control hNPCs were unstained or staurosporine-treated and analysed for apoptosis by flow cytometry as described in Methods. (Bottom) hNPCs, cultured in 5 % oxygen, were mock-infected (left) or BADwt-infected (right, m.o.i. of 3), harvested at 5 days p.i. and analysed by flow cytometry. (b) Quantification of HCMV-induced apoptosis of hNPCs under physiological oxygen tensions. Apoptosis induction and cell viability of HCMV-infected hNPCs were analysed in three independent experiments as in (a). Results of each experiment were averaged based on the population of cells in each quadrant. Results are displayed for mock-infected hNPCs (black) and BADwt-infected hNPCs (white) at 5 days p.i. Analysis is shown as ±sem (n = 3, *P<0.05, **P<0.01, Student’s unpaired t-test). The average values for late (AV⁺/PI⁺) apoptosis and viable cell numbers are listed in the table below.