Fig. 3.

HCMV anti-apoptotic genes are expressed in hNPCs. (a) Transcription of HCMV anti-apoptotic genes in infected hNPCs. hNPCs were mock- or BADwt-infected (m.o.i. of 3) and cultured under low oxygen conditions. Total RNA was isolated from mock- and HCMV-infected hNPCs at the indicated times of infection and analysed by RT-PCR using UL37x1 or β-actin (left) and UL36, UL37x3, UL38, IE1, IE2 and β2.7 primers (right). (b) Kinetics of HCMV IE, early and true late protein production within HCMV-infected hNPCs. hNPCs were mock- (M) or BADwt-infected (H) (m.o.i. of 3) and cultured under low oxygen conditions. Total protein lysates were collected at the indicated times following infection. Total proteins (20 µg) were resolved by SDS-PAGE and analysed by Western blots for IE (pUL37x1/vMIA), early (pp65) and true late (pp28) proteins.