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Regulated temporal HCMV gene expression in BADsubUL37x1-infected hNPCs. hNPCs were mock-, BADwt- or BADsubUL37x1- (m.o.i. of 1) -infected. Total protein was harvested at the indicated times. Total proteins (20 µg) were resolved by SDS-PAGE and analysed by Western blot analysis for IE (IE1/2, pUL37x1/vMIA), early (pp65) and true late (pp28, MCP) proteins. β-Actin was used as a loading control.
