Figure 11. Time course of the xylosyltransferase activity in the microsomes from the wild type, irx9, irx14, and the mutants expressing OsGT43 genes. Microsomes were isolated from stems of 8 independent transgenic lines for each construct. The isolated microsomes were incubated with radiolabeled UDP-xylose and the Xyl4 acceptor, and the xylosyltransferase activity (CPM) was measured by the amount of radiolabeled xylosyl residues transferred onto the acceptor. The data are mean ± SE of 3 technical replicates. (A) Restoration of the xylosyltransferase activity in irx9 by expression of OsGT43A and OsGT43E but not OsGT43H and OsGT43J. (B) Restoration of the xylosyltransferase activity in irx14 by expression of OsGT43J but not OsGT43A, OsGT43E and OsGT43H.