Figure 2. Light stabilizes myc-ACS5 protein, but destabilizes HA-EOL2 protein. Left: Immunoblot of protein extracts from 3-day-old seedlings expressing either a myc-ACS5 (A) or an HA-EOL2 (B) fusion protein after treatment with light. Seedlings were grown in the dark for 3 days, moved to light for the indicated times, proteins extracted and analyzed by immunoblotting using an anti-myc, anti-HA, or an anti-BiP antibody as described.14 Right: The myc and HA signal intensities were quantified and normalized to the BiP loading control.