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. 2013 Dec 5;8(11):e27335. doi: 10.4161/psb.27335

graphic file with name psb-8-e27335-g5.jpg

Figure 5. Expression profiles of CtC4H and CtICS genes after SA treatment of 0.1 mM (A) and 1 mM (B). Samplings were performed at 0, 3, 6, 12, 24, and 48 h after treatment. Total RNA was prepared from all plants followed by DNaseI treatment. Then, RNA was reverse transcribed to cDNA. For CtICS, the internal primers were used for RT-PCR. Intensity of PCR products at different time points refers to as temporal expression level of the genes. 18S rRNA transcription levels serve as internal control.