Table 2.
Human and animal studies on adipose tissue derived stem cell secretome.
| Topic of the study | Source | Pas-sage | Samples | Identification technique | Secreted Protein |
|---|---|---|---|---|---|
| Secretome characterization 51 | Human Liposuction Aspirate | P1 | CM -collected after 16 hours in SFM | 2D Page, tandem mass spectrometry, Western blot | Adiponectin,angiotensin, cathepsin D, pentraxin, pregnancy zone protein, retinol binding protein,CXCL12 |
| Cytokine profile 53 | Human Liposuction Aspirate | P2 | CM -after 72 hours under stimulation EFG, FGF ascorbic acid or LPS | ELISA, RT-PCR | HGF, GM-CSF, ILS, 6,7,8,11,TNFα |
| Immunom-odulation 63 | Human Liposuction Aspirate | P2 and P5 | Supernatants of 5 day old co-cultures (transwell) between MLR and ASCs | ELISA | TGFβ, HGF, PGE2 |
| Inflammatory stimulus 64 | Cambrex Bioscience | P1 | CM - collected after 24 hours of incubationwith ASCs | ELISA | VEGF,HGF, ILG-I |
| Immunom-odulation; biomaterials 65 | Rats -inguinal fat Pad |
P2 | CM - collected after 3 and 6 days over a 6 day culturing period | ELISA | IL-6, VEGF,TNFα |
| Vascularization 66 | Mice-inguinal fat | P0 | Tissue Samples | Immunocyto-chemistry | VEGF |
| Vascularization 67 | Mice-inguinal fat | NR | Cell extracts and CM | Real Time PCR, ELISA | HGF, VEGF, TGFβ. FGF-2 |