Figure 4. C. reinhardtii CDPK1 is localized in the cell body and flagella. (A) Immunostaining of cells expressing CDPK1-GFP with anti-GFP antibody. CDPK1-GFP fusion protein was localized in C. reinhardtii flagella and cell body. (B) Whole cell (WC), cell body (CB), isolated flagella (F), axoneme (Ax), and membrane-matrix (M-M) fractions of CDPK1-GFP clone were analyzed by immunoblotting using anti-GFP antibody. CDPK1- GFP fusion protein was present in the cell body and the membrane-matrix fraction of the flagella. The bar on (A) indicate 10 µm.