Table 1. Primer sequences used in this study.
| Name | Primer Sequence (5′-3′) |
|---|---|
| Primers for sequencing pCCDPK1GFP | |
| C1seqF1 | ACTCGTTGTGCATTCTAGGACC |
| C1seqR1 | AGCTTCAGCGTCTGGTCG |
| C1seqF2 | TGTGGCGTTGCACACAGAT |
| C1seqR2 | TTGTTGGTGGCGGAGTAAGT |
| C1seqF3 | GGACTGCTGGAAGGACTACG |
| C1seqR3 | TCCGACAGCAGGAAGTTCTC |
| C1seqF4 | CACAACATGGGCGTCATTC |
| C1seqR4 | CTGCACCGTACGTCTGCTG |
| C1seqF5 | CCGCAGAGTGTATGACATGG |
| C1seqR5 | ACGCGACGGAAACACATAG |
| C1seqF6 | CTGCGTCCACGCGTCTAC |
| C1seqR6 | TGCCGTTGATCTCGTCCA |
| C1seqF7 | CCTGCTGGTGTGTTTGCAAC |
| C1seqR7 | TCAATCACACGGCACATCG |
| C1seqF8 | GGAAGCGTCTGGTGAAGATG |
| C1seqR8 | GTCTTGTAGTTGCCGTCGTCC |
| C1seqF9 | AGCAGCACGACTTCTTCAAGTC |
| C1seqR9 | CACACCGACCCTGGTCAC |
| Primers for generating CDPK1-GFP | |
|---|---|
| CIGUF | GAGGCCTAGGAGACTGTGCATGTGAGGC |
| C1GR | CATATGGAGGCCTAGGAGACTGTGCA |
| C1GUFNdeI | CAACCCGCGCATCATTGCCACGAA |
| CIGREcorI | GAATTCCAACCCGCGCATCATTGCC |
| GFPfwdEcoRI | GAATTCATGGCCAAGGGCGAGGAGC |
| GFPrevXbaI | TCTAGATTACTTGTACAGCTCGTCCATGCCG |
| Primers for Actin PCR | |
|---|---|
| CreActin fwd | CGCTGGAGAAGACCTACGAG |
| CreActin rev | GGAGTTGAAGGTGGTGTCGT |
| Primers for Real Time PCR | |
|---|---|
| Cblp fwd | CTTCTCGCCCATGACCAC |
| Cblp rev | CCCACCAGGTTGTTCTTC |
| CDPK1RT1F | AGCCAACTTGGAATGAGGTG |
| CDPK1RT1R | CGTCTTGCCCTCTGTAAAGC |
| CDPK3RT1F | TTCTCGGTTGCAAACATCCT |
| CDPK3RT1R | ACGTCCTTCACGTCCTCCTT |