Figure 4. Effect of serum on PNT1A (A- upper left and right) and LNCaP (B- lower left and right) STEAP1 mRNA and protein stability, after treatment with Act D and Chx, respectively.

mRNA and protein fold variation relatively to incomplete medium (IM) were determined by qPCR and Western blot, respectively. mRNA and protein expression was normalized with hGAPDH and with β-actin, respectively. Error bars indicate mean ± SEM of n=6. *p<0.05, **p<0.01 (t- test) comparing (IM) and complete medium (CM) STEAP1 expression at each indicated time.