Table 1. Sequences, amplicons sizes and annealing temperatures for the different STEAP1 mRNA and the internal controls used for quantitative Real-Time PCR.
| Oligo name | Sequence (5ʹ-3ʹ) | Amplicon Size (bp) | Anneling temperature |
|---|---|---|---|
| hSTEAP1_619fw | GGC GAT CCT ACA GAT ACA AGT TGC | 128 | 60°C |
| hSTEAP1_747rv | CCA ATC CCA CAA TTC CCA GAG AC | ||
| hSTEAP1B1fw | CTGGAAGCCTGGTAGCTTTG | 162 | |
| hSTEAP1B1rv | GGCTGGCTGCTGATAAAATG | ||
| hSTEAP1B2fw | CGATTATTTGCAAACAGCCC | 173 | |
| hSTEAP1B2rv | GGGAAGTTGCTAAAGGGTGA | ||
| hGAPDH_74fw | CGC CCG CAG CCG ACA CAT C | 75 | |
| hGAPDH_149rv | CGC CCA ATA CAA TCC G |