Figure 1. Response of OsSUV3 transgenic plants to heavy metal stress. Quantitative real-time PCR analysis of OsSUV3 under different abiotic stress conditions (A) 200 µM CdCl₂ (B) 300 µM ZnCl₂. Total RNA isolation was done from leaf samples collected at different time intervals (viz. One h, 2 h, 3 h, 6 h, 12 h). Bars indicate the standard error (± SE) calculated from three independent experiments. (C) Leaf disk senescence assay of OsSUV3 transgenic rice plants along with antisense (AS), vector control (VC) and wild-type (WT) in presence of 200 µM CdCl₂ and 300 µM ZnCl₂. (D) Estimation of total chlorophyll content of OsSUV3 transgenic plants along with AS, VC and WT plants after CdCl₂ stress. (E) Estimation of total chlorophyll content of OsSUV3 transgenic plants after ZnCl₂ stress. (F) Germination test of T2 SUV3 transgenic AS, VC and WT seeds in MS plate supplemented with 200 µM CdCl₂. (G) Germination of T2 SUV3 transgenic seeds after 300 µM ZnCl₂. (H) Fresh weight per 10 seedlings from MS plate supplemented with 200 µM CdCl₂. (I) Fresh weight per 10 seedlings from MS plate supplemented with 300 µM ZnCl₂.