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. Author manuscript; available in PMC: 2015 Jul 15.
Published in final edited form as: J Immunol. 2014 Jun 16;193(2):610–616. doi: 10.4049/jimmunol.1400556

Figure 1.

Figure 1

NKG2E is translated and associates with CD94, but fails to be expressed on cell surfaces. (A) NKG2C but not NKG2E is expressed on cell surfaces. 293T (left) and Ba/F3 (middle) cells were transfected with MYC tagged NKG2C or NKG2E in conjunction with DAP12 and CD94. Cells were surface stained using anti-MYC mAb and analyzed by flow cytometry. Protein expression was verified by western blot (right). Data are representative of at least three independent experiments. (B) CD94 and DAP12 are expressed on cell surfaces when transfected with NKG2C but not NKG2E. Ba/F3 cells were transfected with CD94 and FLAG-tagged DAP12 alone (left) or additionally with NKG2C (middle) or NKG2E (right) and analyzed for CD94 and DAP12 surface expression by flow cytometry using anti-CD94 or anti-FLAG mAb. Data are representative of at least three independent experiments.