Photomicrographs of coronal sections through the hypothalamus at the level of the paraventricular nucleus of the hypothalamus (PVH) showing a), a Nissl stained section and the cytoarchitecture of the region; b), in situ hybridization (ISH) signal for the vesicular glutamate transporter 2 mRNA; c), ISH signal for the glutamic acid decarboxylase 65 mRNA, which identifies GABAergic neurons; and d) dopamine β-hydroxylase immunoreactivity. a) – c) are from the same animal; d) is from a different animal.
Abbreviations: 3V, third ventricle; AHN, anterior hypothalamic nucleus; fx, fornix; LHA, lateral hypothalamic area; mp, medial parvicellular part of the PVH; ot, optic tract; pm, posterior magnocellular part of the PVH; pv, periventricular part of the PVH; SBPV, subparaventricular zone; vp, ventral parvicellular part of the PVH; ZI, zona incerta;