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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Methods Mol Biol. 2013;1013:93–127. doi: 10.1007/978-1-62703-426-5_7

Figure 5.

Figure 5

Interpretation of curves obtained in a “conventional” BRET titration experiment as proposed in [49]. Variations in the BRETmax suggest a conformational change between the binding partners while variations in BRET50 are interpreted as changes in association affinity. The limits of applicability of this scheme are discussed in text.