Figure 6. C. difficile infection induces IFNγ production by neutrophils, but not T cells.
A) Flow cytometric dot plots of IFNγ versus CD4 in uninfected and infected WT versus IDO1-/- ceca. B) Upper panel, flow cytometric gating of IFNγ-negative (P1) versus IFNγ-positive (P2) cecal populations following C. difficile infection. Lower panels, flow cytometric analysis of CD11b and Ly6G in IFNγ-negative (P1) versus IFNγ-positive (P2) cecal populations. C) Confocal immunofluorescent analysis of MPO (green) and IFNγ (red) in infected WT and IDO1-/- ceca. High power fields (right panels) represent the numbered areas from the low power field image.