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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Connect Tissue Res. 2013 Jul 19;54(0):275–282. doi: 10.3109/03008207.2013.811236

Figure 2. Deficits in matrix collagen associated with TSP2-deficiency are observed as early as day 9.

Figure 2

A: At day 9, collagen levels in conditioned medium (A; left panel, black bars), acid soluble matrix (A; left panel, open bars) and relative levels in acid insoluble matrix (A; right panel) were determined using a Sirius red dye-binding assay. B: Western blot analysis of type I collagen, fibronectin and actin was conducted on detergent soluble cell-matrix protein preparations. Blot is representative of data obtained from material harvested at culture days 7–10 from 3 independent cell harvests. C: DNA levels at day 9 were quantified by UV spectrophotometry. Bars are mean and SD of data obtained from 3 independent cell harvests totaling 12 wells per genotype, # p<0.05.