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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Connect Tissue Res. 2013 Jul 19;54(0):275–282. doi: 10.3109/03008207.2013.811236

Figure 3. In MSC undergoing osteoblast differentiation, TSP2 deficiency does not affect the amount of type I collagen present in conditioned medium.

Figure 3

A: After 4 days in osteoblastic culture, collagen gene expression levels, normalized to actin, were determined by rtPCR. B: Collagen protein levels in cell-conditioned medium were determined by Western blot (top panel). Band intensities were determined using histograms generated in ImageJ (bottom panel). Bars are mean and SD of data obtained from 2 independent cell harvests totaling 6 wells per genotype.