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. 2014 Jul 3;15:12. doi: 10.1186/1471-2199-15-12

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Copyright © 2014 Shigunov et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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DZIP1 is located predominantly in the cytoplasm and shows a granular distribution. (A) Indirect immunofluorescence staining of DZIP1 (green) in HeLa cells. (B) Nuclei counterstained with DAPI (blue) and (C) merged image. HeLa (D-F), HEK293 (G-I) and hTERT-RPE1 (J-O) cells were transfected with pDZIP1-GFP. (J-O) Ciliary axonemes were labeled with anti-acetylated tubulin antibodies (red). (M-O) are magnified sections from (K) (white boxes). Arrow = potentially compatible with a basal body localization. Scale bar: 10 μm.