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. 2014 Jul 3;15:12. doi: 10.1186/1471-2199-15-12

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Copyright © 2014 Shigunov et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Identification of DZIP1-associated mRNAs in HeLa cells. (A) Western blot of HeLa cell proteins immunoprecipitated with a specific anti-DZIP1 antibody and a control IgG, probed with a specific anti-DZIP1 antibody; (B) mRNAs associated with DZIP1. Microarray analysis of the immunoprecipitated RNA fractions. Rows correspond to individual transcripts and the color code indicates the degree of enrichment. Three independent assays, with anti-DZIP1 antibody and negative controls, are shown. (C) Quantitative RT-PCR analysis of transcript levels in DZIP1-IPs (DZIP1 immunoprecipitate) versus negative IPs (IP-Control), normalized to concentration. Enrichment of the following mRNAs was analyzed: IFT80, PUM1, SNX2, BRD8, PTCH, DISP1, NPC1, CSNK1E, MYO5B, CEP164, STK36 and ASH1L. The mean of technical triplicates is shown. (D) Quantitative RT-PCR of transcript levels in DZIP1-GFP IPs versus negative IPs (IP-GFP), normalized to concentration. Enrichment of the following mRNAs was analyzed: IFT80, PUM1, SNX2, BRD8, PTCH, DISP1, NPC1, CSNK1E, MYO5B, CEP164, STK36 and ASH1L. The mean of technical triplicates is shown.