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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Sci Transl Med. 2013 Aug 14;5(198):198ra107. doi: 10.1126/scitranslmed.3006637

Figure 6. HCMV neutralization titers and IgG indexes.

Figure 6

A) Titers of HCMV-neutralizing antibodies from subjects seropositive for HCMV at exam 1 were determined at exams 1-4 by neutralization assay. HCMV strain AD169IE2-YFP was incubated with a 1:100 dilution of serum prior to infection of MRC5 fibroblasts for 16h. YFP signal was quantified and all samples were normalized to a HCMV seronegative control. Mean titers are denoted by the purple line. B) HCMV IgG antibody indexes of individuals who were seropositive at exam 1 were also determined at exams 1-4 by commercial ELISA, using a 1:21 dilution of sera, as per the manufacturer's recommendations. Means are denoted by the blue line. Data were analyzed by one-way repeated measures ANOVA. Statistical differences between means were evaluated using Tukey's multiple comparison's test, where n.s. = not significant.