Figure 1. Longitudinal organisation of V. cholerae chromosome I: Sequential duplication and segregation.

(A) Circular V. cholerae chromosome I and II maps indicating the position of the different tags with respect to oriC1, parS1 and matS sites and their corresponding colour code. (B) Proportion of 2 foci cells according to the realigned cell length (cell size intervals of 0.1 µm) for the different loci of the chromosome I. For each locus, a minimum number of 800 cells were analysed. Left panel: left replichore; Right panel: right replichore. The first cell size interval where ≥50% of cells contained two L3_I foci served to align the cell length distributions of ADV20, ADV21, ADV22, ADV23, ADV25, ADV33, ADV42, ADV50 and ADV51 strains, using ADV24 L3_I as reference. The strain EPV213 was aligned against ADV42 using the timing of recruitment of ter _I to midcell. (C) Reconstitution of the segregation choreographies of the 12 chromosome I loci. Left panel: left replichore; Right panel: right replichore. The median, the 25^th and the 75^th percentiles of the relative cell position of each locus are plotted for each cell size interval. The cells falling into the first interval were named newborn cells and the ones falling into the last interval were named dividing cells. 0: new pole; 1: old pole. (D) The relative distance between different chromosome I loci to the L3I locus was measured as a function of the relative cell length in the cells containing only one focus of each locus. The median (horizontal bar), the 25th and the 75th percentiles (open box) and the 5th and the 95th percentiles (error bars) of the distance of a given locus to L3I were indicated at this locus position along a chromosome I linear genetic map. (E) Relative distance between any of the chromosome sister loci, measured in cells with a length >3.4 µm.