Figure 3. Reorganisation of chromosome I upon parS1 displacement.

(A) Circular map indicating the position of the different loci analysed in different genetic background (WT, parS1 _{300 kb} and parS1 _{650 kb} from left to right, respectively) and the colour code of the analysed loci. (B) Reconstitution of the segregation choreographies, as in Figure 1C. 2 µm of cell elongation were shown centred on the cell interval where at least 50% of the cells had duplicated ori_I. For the WT choreography, ADV114 and ADV78 cell size distributions were aligned with the ADV24 cell sizes using the first cell size interval where 50% or more of cells contained duplicated ori_I. For the parS1 _{300 kb} choreography, CP604 and CP582 cell size distributions were aligned with the CP591 cell sizes using the first cell size interval where ≥50% of cells contained 2 foci of ori_I and L3_I, respectively. For the parS1 _{650 kb} choreography, the cell sizes correspond to strain CP605 (ori_I and ter _I). CP583 and CP586 cell size distributions could not be realigned. The corresponding data was plotted with dashed lines (L2_I and R2I). (C) Relative distances between any of the sister loci, as in Figure 1E. (D) Relative distance between any loci to ter_I locus, as in Figure 1D. (E) Relative distance between ori_I sister loci, in with displaced parS1 sites, as in Figure 1E.