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. Author manuscript; available in PMC: 2015 Aug 1.
Published in final edited form as: Mol Immunol. 2014 May 20;60(2):116–128. doi: 10.1016/j.molimm.2014.04.007

Figure 7. Limitations in the TCR repertoire of γδ T cells responsive to the oxidized dimeric B:9-23 antigen.

Figure 7

Panel A Hybridomas expressing Vγ6Vδ1 fail to respond to dimeric B:9-23

Panel B A hybridoma expressing Vγ5Vδ1 fails to respond to dimeric B:9-23

Panel C Several hybridomas expressing Vγ4 fail to respond to dimeric B:9-23

Panel D High spontaneous reactivity makes it difficult to detect B:9-23 reactivity in most Vγ1+ hybridomas, but hybridoma 77BAS-12 (Vγ1+Vδ6.3+) is a responder.

For all panels, culture conditions and peptide stimulation were as described for Fig.2, panel C. Cellular responsiveness was determined by stimulation with plate-bound anti-CD3ε mAbs, except for panel B (asterisks). Here, soluble anti-CD3ε mAbs were used (10 µg/ml), which provide a weaker stimulus. Responses were measured in triplicate using ELISA for IL-2. Bars show mean response values +/− SE.