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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Nature. 2011 May 19;473(7347):326–335. doi: 10.1038/nature10147

Figure 3. Grafts of human ESC-derived cardiomyocytes in the cryoinjured guinea pig heart.

Figure 3

Representative photomicrographs demonstrating substantial implants of human myocardium within the scar tissue. a, By picrosirius red stain, the scar appears red and viable tissue green. Scale bar=500 μm. b, The human origin of the graft myocardium was confirmed in an adjacent section by combined in situ hybridization with a human-specific pan-centromeric (HuCent, brown) probe and β-myosin heavy chain (βMHC, red) immunohistochemistry. c, Inset from panel b at higher magnification. Note the nuclear localization of the HuCent signal, confirming human origin of these cells. d, Immunostaining for α-actinin highlights the sarcomeric organization of the graft myocytes.