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. 2014 Jun 19;14:170. doi: 10.1186/1471-2229-14-170

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Copyright © 2014 Houshyani et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Constructs used for transgene introduction in Arabidopsis. (a) Genes stacked in COX + and COX++ lines by crossing: HMGR1S, short (cytosolic) isoform of 3-hydroxy-3-methylglutaryl coenzyme A reductase 1; FPS1L, farnesyl diphosphate synthase 1 long isoform; FaNES1, nerolidol synthase 1 from Fragaria x ananassa; COX, mitochondrial signal peptide; P35S, CaMV 35S promoter; Tnos, terminator of the Agrobacterium tumefaciens nopaline synthase. (b) Average signal intensities of the overexpressed (HMGR1S and FPS1L) and housekeeping (GADPH and ACT2) genes in Col-3 (WT), COX + and COX++ lines. Bars indicate the 95% confidence interval.