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Sequence of mCherry. (A) DNA sequence of codon-optimized mCherry gene. The potential translational start sites are indicated in bold and underlined; potential ribosome-binding sites and the stop codon are underlined. The codons mutagenized to stop codons are in red and boxed. (B) Protein sequence of mCherry indicating the four potential start sites in bold. The identified start site is underlined.
