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. Author manuscript; available in PMC: 2015 May 21.
Published in final edited form as: Adv Funct Mater. 2014 Jan 21;24(19):2899–2906. doi: 10.1002/adfm.201303188

Figure 5.

Figure 5

Intracellular delivery of various compounds by targeted nucleodendrimers. (A-B) Fluorescence microscopy of HUVECs co-incubated at 37°C in a pulse-chase mode (see Methods section) with anti-ICAM NDs or anti-ICAM NPs along with (A) biotin-phalloidin or (B) rhodamine-albumin nuclear localization sequence chimera (albumin-NLS). Biotin-phalloidin was visualized with Texas-red streptavidin (note: cell nuclei naturally contain biotin). Arrows = distribution to the intended intracellular targets, including F-actin in (A) or the nucleus in (B). Arrowheads = distribution to intracellular off-target sites, such as lack of F-actin staining in (A) or perinuclear distribution in (B). (C) Fluorescent protein expression 48 h after co-incubation of HUVECs for 5 h at 37°C with anti-ICAM NDs or anti-ICAM NPs and EGFP-RhoA-encoding plasmid. Inset = phase-contrast verifying presence of cells. (A-C) Scale bar = 10-μm.