Fig. 5.

Calculation of PDE activity data from the ITC data. A) Replotting of data as hydrolysis rate vs. substrate by the calorimeter program. Very small and high end numbers are removed due to mixing artifacts. B) Determination of kinetic parameters of PDEs. The truncated data from Fig. 5A is non-linearly fit to the Michaelis-Menten equation: $\mathrm{V}={\mathrm{V}}_{max}\ast (\mathrm{S}/{\mathrm{K}}_{\mathrm{m}}+\mathrm{S})$, where K_m - the Michaelis-Menten constant), V_max - the maximum enzyme activity, S – substrate concentration.

The dashed line corresponds to data obtained during measurements, the continuous line corresponds to fit curve. All reactions were done in triplicate or more.