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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: J Immunol. 2010 Jun 11;185(1):284–293. doi: 10.4049/jimmunol.1000679

Figure 2. DN2b cells are a developmentally intermediate population between DN2a and DN3.

Figure 2

Purified ETP, DN2a, DN2b, and DN3a precursors were plated and co-cultured with OP9-DL1 cells and analyzed for developmental progression. (A) Development of Bcl2 transgenic mouse DN cells after 4 days, showing changes in Kit vs. CD25 (top row) and CD44 vs. CD25 (bottom row). Histograms show direct comparisons of Kit and CD44 surfaces levels between the different cultured populations. (B) Kit vs. CD25 expression for purified DN populations from B6 (top row) and NOD (bottom row) mice, after 4 days of culture. (C) Plots showing development, after 13 days of culture, of NOD and B6 CD4+CD8+ DP cells (top panels), and CD45+ γδTCR+ T cells from the same cultures (bottom panels). Results are representative of 2–4 similar experiments.