Skip to main content
. 2014 Jul 10;10(7):e1004464. doi: 10.1371/journal.pgen.1004464

Figure 3. Overexpression of TOPP4 in topp4-1 rescued the severely dwarfed phenotype, and amiRNA lines of TOPP4 showed dwarfed phenotypes.

Figure 3

(A) Representative 6-week-old 35S-TOPP4 topp4-1 transgenic lines. (B) qRT-PCR was used to detect the relative expression levels of TOPP4 in five 35S-TOPP4 topp4-1 transgenic lines shown in (A). The expression level of TOPP4 in wild type was set to 1.0. (C) Representative 5-week-old amiR-TOPP4 transgenic lines. The first number 1 or 2 of the lines represents transgenic plants generated by amiR-TOPP4-1 or amiR-TOPP4-2, respectively. The white arrows indicate partial-sterile siliques. (D) The height of 5-week-old wild type and three amiR-TOPP4 transgenic lines. (E) qRT-PCR was used to detect the relative expression levels of TOPP4 in three amiRNA lines shown in (C). The expression level of TOPP4 in wild type was set to 1.0. (F) The protein levels of TOPP4 in wild type, topp4-1, and three amiRNA lines of TOPP4. The coomassie brilliant blue-stained RbcS protein was used as loading controls. Scale bars = 1 cm in (A) and (C). Asterisks in (B), (D) and (E) represent statistic differences based on Student's t test with P<0.05. In (B), (D) and (E), error bars represent SE (n = 3).