Skip to main content
. 2014 Jul 10;10(7):e1004495. doi: 10.1371/journal.pgen.1004495

Figure 4. The RNAi knock-down of SFMBT does not affect the abundance of PHO and PHOL.

Figure 4

A. Serial two-fold dilutions of nuclear protein from mock-treated cells and cells treated with dsRNA against SFMBT were transferred to PVDF membrane and probed with indicated antibodies. Arrows indicate the positions of two SFMBT isoforms. B. SDS-PAGE of total nuclear protein followed by coomassie staining was used to control the loading. The weights of molecular standards (in kDa) are shown to the left. As indicated by western blots the RNAi causes roughly four-fold reduction of the SFMBT level, but does not affect the overall abundance of PHOL and causes slight (∼2-fold) reduction of the PHO level. The RT-qPCR analyses indicates that the expression of pho, phol (C) and the set of PcG target genes investigated in this paper (D) is also not altered. In C and D the mean of two independent experiments and the scatter (error bars) are shown.