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. 2014 Jul 10;10(7):e1004476. doi: 10.1371/journal.pgen.1004476

Figure 1. Flow cytometric seed screen on single seeds of Boechera gunnisoniana to analyse ploidy.

Figure 1

Fluorescence intensity was measured on individual green seeds of Boechera to determine the ploidy level of embryo and endosperm. 98% of the seeds measured (N = 84) showed a 3C∶9C ratio of embryo∶endosperm, indicating diplosporous apomixis (A). The remaining 2% showed a 6C∶9C embryo∶endosperm ratio indicative of a BIII hybrid where the embryo is derived from fertilization of an unreduced egg cell (B).