Table 1. Transcriptome analysis of 11 samples from apomictic Boechera isolated by LAM.
Sample | Genes present on microarray with BgPANP (p value≤0.02) | Number of B. gunnisoniana genes with ≥5 read counts | Number of Arabidopsis homologues with ≥5 read counts | Expressed in at least 2 samples (column 1 and column 3) |
apo_initial1 | 5'595 | 6'006 | ||
apo_initial2 | 5'686 | |||
apo_initial3 | 16'385 | 13'047 | ||
sporo_nucellus1 | 5'706 | 4'345 | ||
sporo_nucellus2 | 5'236 | |||
egg_cell1 | 5'835 | 4'490 | ||
egg_cell2 | 17'828 | 13'811 | ||
central_cell1 | 2'973 | 2'192 | ||
central_cell2 | 19'091 | 14'893 | ||
synergid_cell1 | 4'149 | 2'472 | ||
synergid_cell2 | 10'409 | 9'390 |
Summary of gene expression from Boechera germline samples. Samples apo_initial1 and 2, sporo_nucellus1 and 2, egg_cell1, central_cell1, and synergid_cell1 were hybridized on ATH1 microarrays, apo_initial3, egg_cell2, central_cell2, and synergid_cell2 were analysed using RNA-Seq (SOLiD V4) by mapping of reads to the B. gunnisoniana reference transcriptome.