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. 2014 Jul 15;25(14):2152–2160. doi: 10.1091/mbc.E13-12-0749

FIGURE 1:

FIGURE 1:

shRNA-mediated silencing of CP induces morphological changes. (A) CP-ir is robustly decreased in silenced B16F10 cells. Arrowhead indicates CP-silenced cell adjacent to a nontransfected cell. (B) Quantification of CP-ir decrease in silenced cells. For each category, the average CP fluorescence intensity of transfected cells was normalized to that of untransfected cells on the same coverslip that had been imaged and processed in an identical manner. EV, empty vector; CTL, Scramble-transfected cells; KD, CP-silenced cells. A total of 20–40 cells were analyzed for each condition; ***p < 0.001. (C) Filopodial density is increased in CP-depleted cells. A total of 47–69 cells per condition were analyzed across three different experiments and include 1600–3200 filopodia/condition; ***p < 0.001. Controls include eGFP-transfected (eGFP) and Scramble-transfected (CTL) cells. (D) A representative Scramble-transfected cell (left) and a CP-depleted cell (right). Cells are stained with phalloidin to visualize F-actin. Scale bar: 10 μm.

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