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. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Matrix Biol. 2011 May 11;30(0):346–355. doi: 10.1016/j.matbio.2011.04.005

Figure 1. Heparin treatment of primary hAoSMC shows organized elastin fibers.

Figure 1

Adult human aortic SMCs were grown in the absence (A,B) or presence (C,D) of 300 µg/ml heparin for 7 days, then fixed and processed for immunofluorescence microscopy. Non-permeabilized samples were labeled with polyclonal anti-elastin antibody (red), CellTracker Green for cell membrane, and DAPI (blue) for nuclear stain (A,C); or with van Gieson stain for elastin (B,D). Significant extracellular elastin fibers are shown in the heparin treated samples.