Skip to main content
. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Matrix Biol. 2011 May 11;30(0):346–355. doi: 10.1016/j.matbio.2011.04.005

Figure 6. Heparin stimulates elastin mRNA.

Figure 6

15,000 hAoSMCs/cm2 were treated with or without heparin, and samples were harvested at 6 hrs, 24 hrs, 2 days, 4 days, and 6 days. RNA was extracted, reverse-transcribed, and qPCR was used to measure relative amounts of elastin mRNA expression. Each sample was normalized to GAPDH expression, then to elastin expression in the untreated sample at the corresponding time point. Results are given as delta-delta Ct values, and are the average of 3 independent experiments. Standard deviation indicated by error bars. Elastin mRNA levels rise, beginning at 24 hrs post-heparin treatment and remain elevated for the duration of the experiment.