Table 1. Summary of insulin dose curve insulin signaling pathway activation in the DRG and sciatic nerve.

Analyzed results of in vivo insulin signaling in the DRG and sciatic nerve in response to an insulin dose curve indicate that both sensory neuron cell bodies and the peripheral nerve are insulin responsive. Interestingly, differences in downstream signaling may exist between the DRG and sciatic nerve. Data was fit with a sigmoidal dose response curve and analyzed with a 1-way ANOVA and Dunnett’s post hoc. “not converged” indicates that the sigmoidal dose response curve equation was unable to fit the data.

Protein of Interest	Dose Curve EC50		Dose Curve R2value		10000U/kg Insulin-induced fold change as compared to baseline
	DRG	Sciatic Nerve	DRG	Sciatic Nerve	DRG	Sciatic Nerve
Akt (t308)	33.05	16.3	0.71	0.76	13.9***	18.1***
Akt(s473)	131.3	232.1	0.88	0.63	9.2***	30.5*
GSK3β (s9)	not converged	18367.0	not converted	0.62	1.2	2.9***
AS160(t642)	26.4	4.1	0.33	0.43	1.7*	3.0**
mTor (s2448)	39.8	~11.3	0.28	0.34	1.4*	1.6
ERK (Thr202/Tyr204)	not converted	5682.0	not converted	0.24	2.1***	1.8*

^*=p<0.05,

^**=p<0.01,

^***=p<0.001.