Figure 2.

Effect of combined treatment on cell death of colon cancer cells in vitro. (A) Propidium iodide (PI)-stained cells, ^*P<0.05 as compared to 5-FU-treated cells. (B) Annexin V- and PI-stained cells. (C) Mitochondrial membrane potential (Ψmt) of HT-29 cells, ^*P<0.05 as compared to EtOH-treated cells; Valinomycin-treated cells, P<0.05 as compared to control. Dot-plots from representative experiment for selected groups are presented. (D) Expression of active caspase-3, ^*P<0.05 as compared to 5-FU-treated cells. (E) Mean fluorescence channel of cells stained with antibodies against p53, ^*P<0.05 as compared to control. The cells were treated for 48 h with vitamin D compounds at a concentration of 100 nM and with 5-FU; (A,D,E) 100 or (B,C) 200 μg/ml. Calcitriol, 1,25(OH)₂D₃; PRI-2201, calcipotriol. Statistical analysis, Fisher’s test. N=4–5 in each group; mean (± SD) is presented. 5-FU, 5-fluorouracil.