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Expression of CDKIs p21 and p27, and ERKs in HT-29 cells. Cultured HT-29 cells were exposed to the vitamin D compounds at a concentration of 100 nM and/or 200 μg/ml of 5-FU for 48 h. Equal amounts of protein (100 μg) were used. Densitometric analysis of the western blots was carried out using ImageJ 1.46r. Calcitriol, 1,25(OH)2D3; PRI-2201, calcipotriol. CDKIs, cyclin-dependent kinase inhibitors.
