Fig. 6.

Identification of cis-elements involved in the regulation of E1 promoter activities. A, a diagram showing the sequences of the region containing potential transcriptional factor binding sites. The mutated bases (shown in lowercase letters) were constructed to analyze the effect of GATA and HNF3 protein binding sites on the E1 promoter activities. B, various internal deletions were made in the −1428/+72 E1 fragment as shown in A to scan for the elements involved in controlling E1 promoter activities. C, the effect of GATA and HNF3 sites mutation on E1 promoter activities. The quantitative data from triplicate transfections in HepG2 cells was representative of three independent experiments. The promoter activities of different mutated constructs were normalized and relative to those of the wild type (WT).