FACS analysis of CD54 (ICAM-1 protein) and CD83 in primary macrophages (CD14+) and dendritic cells (DC). CD83, which is not induced by JTT, was used as the control to monitor non-specific binding of IgG. (a) CD54 in CD14+ cells. (b) CD83 in CD14+ cells. (c) CD54 in DC. (d) CD83 in DC. Cells were treated with the sample for 24 hours and subjected to FACS analysis. DMSO (vehicle control), JTT (100 μg/mL), LPS (Lipopolysaccharides, positive control, 0.5 μg/mL), Fraction E (Fr. E) (5 μg/mL).