Skip to main content
. Author manuscript; available in PMC: 2014 Jul 10.
Published in final edited form as: Arthritis Rheum. 2013 Feb;65(2):408–417. doi: 10.1002/art.37768

Figure 2.

Figure 2

Significant decline of superficial zone protein (SZP) accumulation upon blocking of hedgehog signaling with cyclopamine (Cyc), an inhibitor of Smoothened. Primary cells were pretreated with cyclopamine (1 µM or 10 µM) for 3 hours before addition of Sonic hedgehog (Shh) (1 µg/ml) and cultured for 4 days as monolayers in serum-free chemically defined medium. SZP accumulation in spent medium was quantified according to enzyme-linked immunosorbent assay. The up-regulation of SZP accumulation by 1 µg/ml Shh was reduced by cyclopamine in part by 1µM cyclopamine and completely abolished by 10 µM cyclopamine. However, cyclopmaine alone showed no influence on SZP accumulation (Figure 5). Values are the mean ± standard deviations, with n=6 (*p<0.01).