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Accumulation of β-catenin by Wnt-3a or BIO. After treatment with Wnt-3a (100 ng/ml) or BIO (1 µM) for 6 hours, whole cell lysates were collected and analyzed by immunoblot. Treatment with Wnt-3a (100 ng/ml) or BIO (1 µM) resulted in accumulation of cytosolic β-catenin protein. Results shown are representative of 3 individual experiments.
