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The total RNA was extracted from control and ActRIIB/Fc treated myoblasts incubated during 2 days without or with recombinant myostatin (500 ng/ml) in the culture medium. The mRNA expression of MyoD (a), Myf5 (b), myogenin (c) and MRF4 (d) was quantified by Q-RT-PCR. ActRIIB/Fc significantly increased the expression of MyoD and myogenin. The data presented are means + SD (n = 3), *p <0.05.
