Figure 2.

Metformin enhances 5-FU sensitivity on EC cells. Esophageal cancer cells SKGT-4, JHESO, OACP and YES-6 were seeded in 96-well plates and treated with metformin alone at indicated dosage, 5-FU alone at 10 μM and the combination of both for different time points. A non-radioactive MTS cell proliferation assay was done to determine the rate of proliferation (cell survival). The experiments were performed in triplicate and repeated twice; the results reported here are the mean of all these experiments.